Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on cell viability of ( A ) NHF; ( B ) HaCaT; ( C ) HFDPC. The cells were exposed to various concentrations (0–4000 µg/mL) of PPE for 24 h. Data are shown as mean ± SD ( n = 3). *, ** represent a significant difference from DMEM group (a negative control) at p -values of <0.05 and 0.001, respectively.

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques: Negative Control

Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on the proliferation of ( A ) NHF, ( B ) HaCaT, and ( C ) HFDPC cells. Each cell was treated with various concentrations (0–2000 µg/mL) of PPE for different time intervals: 24 h ( ■ ), 48 h ( ■ ), and 72 h (☐). Data are presented as mean ± SD ( n = 3). * and ** represent the significant difference in % cell proliferation compared with the cell proliferation at 24 h at p -value < 0.05 and 0.001, respectively.

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques:

Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on the aggregation of HFDPC cells after treatment for 72 h. HFDPC cells were treated with ( A ) DMEM (control group) and ( B ) 1000 µg/mL PPE. The images were photographed using an inverted microscope (10× objective lens, bright field).

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques: Control, Inverted Microscopy